Water pollution caused by waste chrome leather tanning industry at Sukaregang, Garut Regency should be done by phytoremediation technique using plants from Ponteridaceae family. This research needs to know the level of absorption of Ponteridaceae plants and determine which one is the most effective to absorb chrome. The phase of this research is by gathering materials and determination of the Ponteridaceae plants, waste treatment with phytoremediation in 21 days, and analysis of chromium levels with an atomic absorption spectrophotometer. The absorption capacity of Eichhornia crassipes Solm., Heteranthera peduncularis and Monochoria vaginalis are 1.5395; 0.5728, and 0.1057 µg/g. Based on the Duncan test, it is concluded that Eichhornia crassipes Solm. is the most effective among them with the highest ability to absorb metal chrome tannery wastes. Keywords: Chrome, Eichhornia crassipes Solm., Heteranthera peduncularis, Monochoria vaginalis, phytoremediation | Permasalahan pencemaran air yang disebabkan oleh limbah krom industri penyamakan kulit di kawasan Sukaregang, Kabupaten Garut perlu diolah dengan teknik fitoremediasi dengan tanaman dari keluarga Ponteridaceae. Penelitian ini perlu dilakukan untuk mengetahui kadar penyerapan tanaman eceng-ecengan dan menentukan eceng mana yang paling efektif menyerap krom. Penelitian ini dilakukan dengan tahap pengumpulan bahan dan determinasi tanaman eceng-ecengan, pengolahan limbah dengan fitoremediasi selama 21 hari, dan analisis kadar krom dengan spektrofotometer serapan atom. Kapasitas penyerapan Eichhornia crassipes Solm., Heteranthera peduncularis, dan Monochoria vaginalis adalah 1,5395; 0,5728; dan 0,1057 µg/g. Berdasarkan uji Duncan, disimpulkan bahwa Eichhornia crassipes Solm. merupakan tanaman eceng paling efektif yang memiliki kemampuan paling tinggi dalam menyerap logam krom limbah penyamakan kulit. Kata kunci: Eichhornia crassipes Solm., fitoremediasi, Heteranthera peduncularis, krom, Monochoria vaginalis

Protein-based therapies have already brought about a significant transformation in the field of medicine, and their use continues to grow. The development of protein therapeutics has revolutionized the treatment of various diseases, such as cancer, autoimmune disorders, viral infections, cardiovascular disease, myocardial infarction, etc. Consequently, it is crucial to ensure the stability of formulations, given their rapid growth. Preserving the structural integrity of protein-based medicines is a significant challenge in developing a stable and high-quality formulation. This challenge arises during various stages, including manufacturing procedures, storage, handling, distribution, and delivery due to proteins' intricate and delicate nature. Hence, it is crucial to thoroughly understand the various degradation mechanisms that impact protein stability to enhance different variables and minimize the formation of degradation products, such as aggregation, oxidation, deamidation, etc., which could potentially have clinical implications. This review provides an important step in understanding the process of protein degradation and offers a beneficial approach to investigate the degradation of proteins, specifically aggregation, through several analytical and biophysical methods. This is important for facilitating the further advancement of protein-based therapies.

Bawang merah (Allium cepa L.) selain digunakan sebagai bumbu penyedap masakan, juga bisa digunakan sebagai obat oleh masyarakat Indonesia. Bagian tumbuhan yang dapat dimanfaatkan sebagai obat adalah bagian umbinya. Umbi bawang merah mengandung metabolit sekunder yaitu fenolik, flavonoid, dan terpenoid yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri fraksi n-heksana, etil asetat, dan 1-butanol umbi bawang merah terhadapbakteri Gram positif Staphylococcus aureus dan bakteri Gram negatif Escherichia coli dengan metode difusi cakram. Fraksi umbi bawang merah dibuat dalam beberapa seri konsentrasi yaitu 25%; 12,5%; 6,25%; 3,125%; dan 1,5625%. Konsentrasi 25% memberikan daya hambat paling baik dalam menghambat bakteri Staphylococcus aureus dan Escherichia coli. Hasil analisisdata menggunakan uji one way ANOVA dan Kruskall-Wallis didapatkan hasil p <0,05 yang menandakan adanya perbedaan daya hambat yang signifikan yang diberikan antara seri konsentrasi masing-masing fraksi dengan kontrol positif dan kontrol negatif. Simpulan dari penelitian ini adalah fraksi etil asetat memiliki daya hambat yang lebih besar terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli dibandingkan fraksi n-heksana dan 1-butanol.

The small particle size of nano calcium from freshwater mussels shell will tend to increase the absorp- tion of calcium by human’s body and will be very well consumed by children with special needs to suppress hyperactivity disorders that caused by mercury. This study aimed to create and determine the best instant granule formulation containing nano calcium from freshwater mussels shell. The research method began with the manufacture of freshwater mussels shell powder and isolated nano calcium. Nano calcium was tested for its solubility in acid. The granule formula was made with stevia varia- tions. Instant granule evaluation included granule ow test, rest angle, compressibility, water content, solubility, hedonic test, and calcium bioavailability. The results showed that nano calcium was soluble in 1:1 ratio of citric and tartrate acids, so that each formula needs 1.2% citric and 1.2% tartrate acids. Based on the avor, aroma, and texture, the most preferred granule formula contained 600 mg nano calcium, 60 mg citric acid, 60 mg tartaric acid, 400 mg stevia each sachet weighing 5 g. The best in- stant granule evaluation showed that the ow rate was 4.253 g/sec, 33.90 rest angle, 4.65% compress- ibility, 2.21% water content, soluble time 58 second and calcium bioavailability was 100%. Key words: Calcium bioavailability, detoxi cation, freshwater mussels, instant granule, nano cal- cium.

Metabolic syndrome is associated with an increased risk of hypertension, diabetes, and cardiovascular disease, which are often triggered by oxidative stress and inflammation. This study evaluated the effects of Kaempferia galanga L extract (ERK) on oxidative stress and metabolic dysfunction using a fructose-induced male Wistar rat model. A total of 25 rats were divided into five groups: normal control, positive control, allopurinol group, and two ERK treatment groups at doses of 50 and 100 mg/kg body weight. All groups, except the normal control, were given 25% fructose in the drinking water and an injection of potassium oxonate (4.5 mg/kg body weight) for 28 days. Evaluation was done by measurement of glucose, triglyceride, malondialdehyde (MDA) and nitric oxide (NO) and histological analysis of aortic tissue. The results showed that ERK, especially at a dose of 100 mg/kg, significantly reduced glucose, triglyceride and MDA levels and increased NO levels. ERK administration also reduced the body weight of mice and prevented the formation of foam and inflammatory cells in aortic tissue. The total flavonoid content of ERK also supports its biological activity. This study concludes that ERK has potential as a natural therapeutic agent to treat metabolic syndrome through antioxidant and anti-inflammatory mechanisms.

Cincau hijau (Premna oblongifolia Merr) banyak tumbuh di Indonesia dan merupakan makanan tradisional Indonesia yang kaya akan serat dan memiliki aktivitas antioksidan. Tujuan penelitian adalah untuk mengetahui pengaruh ekstrak etanol daun cincau terhadap penyembuhan maag. dan komposisi butiran mukoadhesif yang optimal. Tikus dikelompokkan menjadi kontrol negatif, kontrol positif, dan 3 kelompok dosis uji. Pengobatan diberikan selama tiga hari, tukak gastrointestinal diberi skor berdasarkan kuantitas dan tingkat keparahan tukak, dan statistik dibandingkan antar kelompok perlakuan menggunakan uji ANOVA satu arah. Hasil penelitian menunjukkan bahwa uji dosis ketiga memiliki aktivitas dalam penyembuhan tukak lambung dibandingkan dengan kelompok kontrol negatif, dengan proporsi penyembuhan luka pada dosis 1 sebesar (12,13%; p. 0,024), dosis 2 sebesar (20,93%; p. 0,004). ), dan dosis 3 menjadi (24,47%; p. 0,000). Oleh karena itu, dapat disimpulkan bahwa ekstrak etanol daun cincau hijau dosis ketiga dipilih sebagai dosis optimal untuk pengobatan tukak lambung. Formulasi terbaik terdapat pada formula 3.

Ambon banana peel (ABP) is rich in phenolic compounds yet unstable to light and heat. Foam-mat drying (FMD) protects bioactive components and facilitates the development of nutraceutical products. This study aimed to formulate and evaluate FMD granules containing ABP for their antioxidant and antihypertensive activities. Granules were prepared with ABP at 1% (F1), 2.5% (F2), and 5% (F3) w/w, combined with 6% Tween-80 and 15% maltodextrin, dried at 40 °C for 60 min, and blended with excipients. Physical characteristics, pH, flowability, and antioxidant activity (ABTS assay) were assessed. Antihypertensive effects were tested in Wistar rats induced with 2% NaCl and treated orally for 14 days; captopril (5 mg/kg BW) served as a positive control. Data were analyzed by one-way ANOVA. All formulations produced free-flowing granules with a moisture content <2% and a dissolution time <5 min. Antioxidant inhibition increased with ABP content, with F3 showing the highest activity (71.3 ± 1.4%). F3 also achieved the greatest reductions in systolic (≈31%) and diastolic (≈39%) blood pressure, approaching the effect of captopril. Moreover, F3 exhibits strong antioxidant activity and significant antihypertensive effects, indicating potential as a sustainable nutraceutical for the management of hypertension. Further studies on product stability and clinical efficacy are warranted.

99mTc-ethambutol is a radiopharmaceuticals used to determine the location of Mycobacterium tuberculosisthat causes TB disease. To prepare this radiopharmaceuticals, several supporting compounds are required in addition to ethambutol and radionuclide compounds. These are mannitol as filler, SnCl2as a reducing agent and sodium pyrophosphate as co-ligand. The radiopharmaceuticals is made in the form of a lyophilized kit without 99mTc radionuclides. The aim of this study was to determined the effect of reducing agents on 99mTc-ethambutol preparation using the High Performance liquid chromatography (HPLC) equiped with radiodetector (radio-HPLC) to the quality of radiopharmaceuticals. In the previous study, the determination of radiochemical purity only used TLC by determining the amount of impurities TcO2and TcO4. This study was carried out more profoundly by using radio-HPLC to ensure the quality of radiopharmaceutical products that were made is actually 99mTc-ethambutol. The Test were performed using paper chromatography, electrophoresis, radio-HPLC, and stability test of 99mTc-ethambutol radiopharmaceuticals. The results showed that the radiopharmaceuticalkit of ethambutol is performing good physical characterictics and high radiochemical purity of 99mTc-ethambutol both in analysis using paper chromatography, electrophoresis and radio-HPLC. The 99mTc-ethambutol shown the peak at retention time (tr) 7,97 minutes using UV and radioactive detector, C18 column, isocratic system using phosphate buffer 0,2 M pH 7,4 and Acetonitrile (90 :10), flow rate 0,8 mL/min. The radiochemical purity of 99mTc-ethambutol is still remain high (91.44 ± 0.92%) after 27 days of kit were made.Key words: 99mTc-ethambutol, radio-HPLC, TB disease, Mycobacterium tuberculosis, radiopharmaceuticals.

Allium ascalonicum (shallot) is a medicinal plant with promising potential in the management of type 2 diabetes mellitus (T2DM). This review summarizes the phytochemical constituents, mechanisms of action, and existing research gaps that warrant further exploration for targeted drug development. A systematic literature search was performed using PubMed, ScienceDirect, and Google Scholar to identify relevant studies published between 2013 and 2023. Only full-text original research articles in English were included, while reviews and incomplete studies were excluded. The search strategy employed the keywords: “Allium ascalonicum OR shallot AND α-glucosidase.” The findings indicate that A. ascalonicum demonstrates significant antidiabetic activity across in vitro, in vivo, and limited human studies, primarily through inhibition of the α-glucosidase enzyme. This activity is largely attributed to quercetin and other flavonoid compounds, which play an essential role in reducing blood glucose levels and mitigating oxidative stress. Collectively, the evidence highlights the therapeutic potential of A. ascalonicum as a natural α-glucosidase inhibitor. However, further clinical investigations and comprehensive toxicity assessments are required to establish its efficacy and safety as a standardized herbal medicine and future phytopharmaceutical candidate for diabetes management.

Diabetes mellitus merupakan penyakit kronis yang berkaitan dengan gangguan metabolisme glukosa darah. Black Garlic memiliki potensi sebagai antidiabetes karena mengandung berbagai senyawa yang dapat mengontrol kadar gula darah seperti asam fenolik, senyawa flavonoid dan S-allyl-L-cysteine (SAC). DPP-4 merupakan enzim yang berperan penting sebagai target terapeutik dari gliptin dalam pengobatan diabetes mellitus mellitus tipe II. Penelitian ini dilakukan dengan tujuan membuktikan potensi senyawa pada Black Garlic sebagai inhibitor enzim DPP-4 secara in silico melalui metode penambatan molekuler. Berdasarkan hasil penelitian, SAC memiliki nilai energi ikatan sebesar -5,36 kkal/mol dan nilai konstanta inhibisi sebesar 118,08 μM. Hal ini menunjukkan bahwa ikatan antara senyawa SAC dengan enzim DPP-4 bersifat stabil dan terbukti berpotensi sebagai inhibitor enzim DPP-4 dibandingkan keempat senyawa uji lainnya. Namun, senyawa SAC tidak lebih baik dari ligan alaminya karena memiliki nilai energi ikatan dan konstanta inhibisi yang lebih kecil. Oleh karena itu dapat disimpulkan bahwa senyawa SAC pada Black Garlic tidak lebih efektif dibandingkan dengan ligan alaminya yaitu Vildagliptin tetapi tetap dapat berpotensi sebagai inhibitor enzim DPP-4.

Inflammation is a self-protection response to begin the healing process. One of the anti-inflammatory targets worth developing is lipoxygenase inhibitors, which have been studied for several diseases, including severe airways disease. The aim of this study was to predict the affinity of 23 compounds that contained in Hemigraphis alternata leaves against 5-lipoxygenase (5-LOX). The compounds of Hemigraphis alternata leaves were screened for its affinity againts 5-LOX using docking software, DOCK 6.9, with zileuton as the comparator. Based on the grid score, most of the 23 of Hemigraphis alternata leaves compounds showed a higher affinity towards 5-LOX compare to zileuton. The highest affinity was shown by n-hexadecanoid acid. The study showed that Hemigraphis alternata leaves contains potential active components that could be developed as 5-LOX-inhibitor.Keywords: In silico, Hemigraphis alternata, lipoxygenase, anti-inflammatory

Basil is one of medicinal plants in Indonesia that has been used empirically as antimicrobes, analgetic, antiinflammatory, antivirus, antitumor, and antioxidants that prevented ischemia. This research aimed to investigate the effect of adding granule ethanol extract of basil leaves (Ocimum americanum) as antioxidant to capture free radicals in fried foods. The antioxidant activity of ethanol extract of basil leaves with DPPH determinated by IC50, comparing the antioxidant activity of the granule and the control by determination of quality properties of oil and food after frying using Fourier Transfor Infra Red (FTIR) spectra, and quantitative parameters by determination percentage of free fatty acid (FFA). Results of maceration with ethanol 15.94% w/w, total ash content 9.85%, water soluble ash content 3.98%, acid insoluble ash content 3.98%, water soluble extract 25.89%, ethanol soluble extract 12.76%, water content 6.87%, drying shrinkage 11.19%. Testing the antioxidant activity of the ethanol extract of basil leaves with DPPH gave IC50 of 80.55 ppm. The result of antioxidant testing by determination percentage of FFA statistically with ANOVA (significance 0.00<5) and comparison of the concentration extract in basil granule with LSD test significantly different. The result indicates that adding ethanol extract of granules basil on fried foods gave antioxidant activity because it can inhibit the increasing of FFA percentage. Keywords: Antioxidant, basil leaves, DPPH, granule

Teak leaves (Tectona grandis Linn. F) are one of the plants that have empirically been used as antifungal, antioxidant, antibacterial, and antidiabetic agents, due to their content of secondary metabolite compounds including saponins, tannins, alkaloids, and sterols. This research aims to determine the secondary metabolite compounds present in the extract and fractions of teak leaves (Tectona grandis Linn. F) and the antifungal activity of the teak leaf extract and fractions against Candida albicans using the microdilution method. The ethanol extract was fractionated using a partition method with solvents n-hexane, chloroform, and ethyl acetate. Antifungal testing was carried out against Candida albicans using the Broth Microdilution method to measure the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) values. Based on the phytochemical screening results, teak leaves contain alkaloids, phenolics, flavonoids, and terpenoids. The MIC results for the antifungal activity of the ethanol extract, n-hexane fraction, chloroform fraction, and water fraction against Candida albicans were all >500 µg/mL. In the meantime, the MIC and MFC values for ketoconazole 200 mg against Candida albicans were 64 µg/mL. These results indicate that there is a difference in MIC and MFC values between the samples and the positive control (ketoconazole 200 mg), with the antifungal activity of the samples being weaker compared to the positive control

Inflammation is a normal protective response to tissue injury that involves various physiological processes in the body. Perisbivalvin, apioside, and pelargonidine 3-sambubioside are anthocyanin compounds found in magenta plants. The purpose of this study was to obtain a candidate for a new compound as an anti-inflammatory agent targeting the PTGS2 receptor before in vivo testing. Molecular docking in silico with PDB code 5IKR was carried out by optimizing 2 and 3-dimensional chemical structures, method validation, and docking between perisbivalvin compounds and the comparison compound mefenamic acid. The results obtained were docking perisbivalvin -7.63 kcal/mol, apioside -0.77 kcal/mol, and pelargonidine -5.74 kcal/mol, while the bond energy of the comparison compound was -7.52 kcal/mol through hydrogen bonding with amino acids TYR 385A and SER 530A. The prediction results of the test compounds and comparison compounds were classified as class 4 toxicities. Perisbivalvin compounds have anti-inflammatory potential because they can bind to the PTGS2 protein.

Zea mays L. (Jagung) adalah tanaman yang banyak dibudidayakan di Indonesia dan pemanfaatan limbah dari jagung masih sebatas sebagai pakan ternak, sedangkan pemanfaatan kandungan komponen didalamnya masih sangat terbatas. Kandungan kimia yang terdapat pada tanaman jagung antara lain alkaloid, saponin, tanin, flavonoid, fenol, steroid, glikosida, terpenoid, protein, mineral. Senyawa fenol banyak berperan dalam aktivitas antioksidan dan tabir surya. Sampel tongkol dan rambut jagung diduga berpotensi sebagai sumber antioksidan dan tabir surya alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan tabir surya dari ekstrak dan fraksi tongkol dan rambut jagung serta kadar fenol totalnya. Uji aktivitas antioksidan dilakukan dengan menggunakan metode peredaman radikal bebas 1,1 Difenil-1-Pikrihidrazil (DPPH) dan uji aktivitas tabir surya dengan penentuan nilai sun protected farctor (SPF) dengan spektrofotometri yang diukur pada λ 290-320nm. Penetapan kadar fenol total diukur dengan metode kolorimetri menggunakan reagen Folin Ciocalteu dan diukur dengan spektrofotometri pada λ 765nm. Hasil pengujian aktivitas antioksidan dan tabir surya terbaik ditunjukkan oleh fraksi etil asetat rambut jagung dengan nilai IC50 sebesar 45,18 μg/mLdan nilai SPF sebesar 23,943 serta kadar fenol totalnya sebesar 106,010 mg/G ± 0,431. Fraksi etil asetat rambut jagung memiliki aktivitas antioksidan, tabir surya, dan kadar fenol yang lebih tinggi dari yang lainnya.

Parijoto fruit (Medinilla speciosa) contains flavonoid compounds that are potentially immunomodulators because they increase macrophage phagocytosis activity and lymphocyte proliferation in vitro. This study aims to determine the phytochemical compound content of parijoto fruit and its potential as an immunomodulatory agent in vivo. Parijoto fruit was extracted using 70% ethanol solvent. The ethanol extract of parijoto fruit (EEPF) was qualitatively identified by phytochemical screening. The immunomodulatory activity test in vivo was conducted using BALB/c mice, which were separated into five groups: untreated, Phyllanthus niruri L. (PN) extract (positive control), and test groups (125mg/KgBW; 250mg/KgBW and 375mg/KgBW). The treatment duration was 18 days, with the induction of the hepatitis B vaccine on days 7 and 14. An immunomodulatory activity test was conducted on the 19th day, which included a macrophage phagocytosis activity test, lymphocyte proliferation, and measurement of IgG levels. The identification results showed that EEPF contains flavonoids, tannins, and saponins. Macrophage phagocytosis activity showed that EEPF increased macrophages' phagocytosis capacity (PC) and phagocytosis index (PI). EEPF increased lymphocyte proliferation activity and IgG levels, significantly different from control and not substantially different from positive control. Collectively, EEPF increases immunomodulatory activity in vivo.

Epiphyllum oxypetalum (DC.) or Wijaya Kusuma (Cactaceae) is an ornamental plant which is knownto contain polyphenol compounds. The purpose to determine the levels of total phenolic and flavonoidcompounds in the fractions of Wijaya Kusuma leaves using microplate-based assay. Each sample wasdetermined phenolic content with Folin-ciocalteu reagent and flavonoid content using AlCl3 on themicroplate. Absorption was measured using a microplate reader. Screening of DPPH radical scavengingactivity was identified using the TLC method. The results showed that the extract and fractions ofthe leaves of Wijaya Kusuma contained phenolic and flavonoid compounds. The ether fraction hadthe highest phenolic content (35,912±0,54776 mgGAE/g) and flavonoids (0,773±0,008 mgQE/g)compared to other fractions (ether>water>ethyl acetate fraction). There is one yellow spot with an Rfvalue of 0,85 in the ether fraction which is thought to be a compound capable of scavenging the DPPHradicals. The ether fraction of Wijaya Kusuma leaves has potential as a source of polyphenols withantioxidant activity. However, further research is needed to evaluate the antioxidant capacity in theether fraction of Wijaya Kusuma leaves.Keywords: Antioxidant, Epiphyllum oxypetalum (DC.) Haw, Flavonoids, Fraction, High-throughputMicroplate Assay, Phenolics.

Kencur (Kaempferia galanga L.) is a family of Zingiberaceae. Several studies have shown that kencur can help reduce inflammation because kencur is known to contain anti-inflammatory compounds, namely marker compounds from flavonoids, kaempferol. For the development of pharmaceutical preparations, research on anti-inflammatory plasters containing 96% ethanol extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from ginger rhizome with the addition of penetration enhancer (enhancer), namely propylene glycol. This anti-inflammatory plaster was tested for its activity in 5 groups of Wistar strain rat feet which had been induced 1% carrageenan (negative control); positive control (diclofenac sodium), ethanol96% extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from kencur rhizome and compared with plaster of kencur rhizome ethanol extract without enhancer. The results showed the effect of adding enhancers 30 minutes after administration. 96% ethanol extract and ethyl acetate extract had reduced inflammation by 79.99% in rat test animals compared to plaster ethanol extract of rhizome kencur without the addition of enhancers. Keywords :  Kaempferia galanga. L., patch, anti-inflammatory, enhancer, propylene glycol

Basil (Ocimum sanctum L.) have a dominant content of citral compounds with antibacterial activity. Another plant suspected to contain antibacterial bioactive components is orange peel. Orange peel (Citrus sinensis L) contains phenolic compounds (phenolic acids, flavonones, and polymethoxylated flavones), carotenoids, and ascorbic acid. Phenolic compounds are known to have several activities such as antimicrobial, antioxidant, anticancer, anti-inflammatory, and anti- allergic. The aim of this research is to prove that the combination of orange peel and basil extracts has antibacterial effects against Staphylococcus aureus, which can be formulated as paper soap which is named O-Citri. This research method is experimental, involving the physical and chemical evaluation of the formulation, as well as antibacterial activity testing. The research results show that the combination of orange peel and basil extracts in the paper soap formulation has antibacterial effects. The highest concentration combination for antibacterial activity is F1 with a ratio of orange peel and basil extract at 5%:15%.

Ursodeoxycholic acid is an active compound to treat cholesterol gallstones which is included in Biopharmaceutical Classification System class II. Ursodeoxycholic acid capsule formulations have been developed by the pharmaceutical industries. Due to requirement, active ingredient testing and dissolution tests need to be routinely carried out to control quality of the drug. Therefore, aim of this study was to develop a simultaneously analytical method for assay and dissolution test. Optimum condition of analysis were using HPLC-RID, C18 column (5 µm; 3.9 x 300 mm), flow rate 1 mL/min, 50 µL volume injection, acetonitrile:buffer phosphate pH 3.0 (55:45 v/v) as mobile phase, column, detector, and sample temperature were 40 oC. This method provides good linearity in a range of 50-130 % concentration for assay and dissolution test with correlation coefficient values of 1 and 0.9995, respectively. LOD and LOQ for assay were in value of 4.892 µg/mL and 14.824 µg/mL whereas for dissolution tests in a value of 6,501 µg/mL and 19,701 µg/mL. The average percent recovery for assay and dissolution test were in a range of 100.018±0.888% and 98.936±2.124 %. Repeatability and intermediate precision of assay were obtained by determining RSD values of 0.466% and 0.279-0.483 %, while the dissolution test were 1.137% and 0.032-0.289 %. The validation show this method are linear, selective, accurate, and precise.Keywords: Assay, dissolution test, Ursodeoxycholic acid, validation